Betaine is accumulated via transient choline dehydrogenase activation during mouse oocyte meiotic maturation

H1foo is indispensable for meiotic maturation of the mouse oocyte.

Oocyte-specific linker histone H1foo is localized in the oocyte nucleus, either diffusely or bound to chromatin, during the processes of meiotic maturation and fertilization. This expression pattern suggests that H1foo plays a key role in the control of gene expression and chromatin modification during oogenesis and early embryogenesis. To reveal the function of H1foo, we microinjected antisens...

Mitofusin-2 is required for mouse oocyte meiotic maturation

Mitofusin-2 (Mfn2) is essential for embryonic development, anti-apoptotic events, protection against free radical-induced lesions, and mitochondrial fusion in many cells. However, little is known about its mechanism and function during oocyte maturation. In this study, we found that Mfn2 was expressed in the cytoplasm during different stages of mouse oocyte maturation. Mfn2 was mainly associate...

Differing roles of pyruvate dehydrogenase kinases during mouse oocyte maturation.

Pyruvate dehydrogenase kinases (PDKs) modulate energy homeostasis in multiple tissues and cell types, under various nutrient conditions, through phosphorylation of the α subunit (PDHE1α, also known as PDHA1) of the pyruvate dehydrogenase (PDH) complex. However, the roles of PDKs in meiotic maturation are currently unknown. Here, by undertaking knockdown and overexpression analysis of PDK paralo...

28: Can Phthalates Disrupt Oocyte Meiotic Maturation in Mouse Model?

Background and aims: Phthalates, which are commonly used to render plastics into soft and flexible materials, have also determined as developmental and reproductive toxicants in human and animals. The purpose of this study was to evaluate the effect of mono-(2-ethylhexyl) phthalate (MEHP) and di-(2-ethylhexyl) phthalate (DEHP) oral administration on maturation of mouse oocytes, apoptosis and ge...

Mitochondrial Dynamics During Mouse Oocyte Maturation